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Nitrosylation of cystein 10 promotes dsNNTTR formation Our results demonstrate that nitrosative thiol modification efficiently promotes intermolecular disulfide bond formation in TTR, as evidenced by the prominent dsNNTTR dimer observed by non-reducing SDSPAGE following GSNO treatment

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After centrifugation at 500g for 15 min ( n = 3) were collected with the number of frozen-thawed spermatozoa adjusted to a final concentration of 1 10 6 sperms/ml, added to the above fertilization droplet containing MII oocytes (repeated in triplicate, 205 oocytes for the NC group, 210 oocytes for the GSH group, 220 oocytes for the SeNPs group and 217 oocytes for the GSH + SeNPs group, repeated in triplicate) and cultured in a CO 2 incubator (38.5C, 5%CO 2 and 5%O 2 ) for 18 h